Tyler McCullough, M.S.

Tyler and MacAcademic Title

Graduate Research Assistant

Contact Information

phone: (352) 273-7413
email: tytym2@ufl.edu

Lab

R2-236/240

About

A native Floridian, Tyler received his Bachelors of Science in Microbiology and Cell Science from the University of Florida in 2011. Shortly after working as a research assistant for Dr. Thomas Conlon on the Glycogen Storage Disease Project, he was accepted into the Masters of Health Science program at the Johns Hopkins School of Public Health. After graduating in 2013 he was fortunate to receive a position as an Oak Ridge Institute for Science and Education research fellow, working with the Food and Drug Administration at the National Institutes of Health in Bethesda. Tyler was focused on evaluating antiviral agents targeting Vaccinia infections in immunocompromised models of mice. While in Maryland, Tyler also began to develop a long held passion for vision (once sparked by his own discovery of being colorblind) by volunteering at the Wilmer Eye Institute in Dr. Shannath Merbs’ lab. His interest in vision research continued to flourish after he was began his appointed laboratory scientist in Dr. Shannon Boye’s lab back at the University of Florida. Though he occasionally misses being closer to snowboarding, warm winters and surfable waves usually helps ease his pain. The focus his thesis research is optimization of Crispr/Cas9 technology for the treatment of inherited retinal disease.

Publications

  • Sanford L. Boye, Elena V. Olshevskaya, Igor V. Peshenko, K. Tyler McCullough, Shannon E. Boye, and Alexander M. Dizhoor. Functional study of two biochemically unusual GUCY2D Leber congenital amaurosis-linked mutations expressed via AAV in mouse retinas. Molecular Vision, in press
  • Boye SL, Peterson JJ, Choudhury S, Min SH, Ruan Q, McCullough KT, Zhang Z, Olshevskaya EV, Peshenko IV, Hauswirth WW, Ding XQ, Dizhoor AM, Boye SE. Gene Therapy Fully Restores Vision to the All-cone NRL-/-Gucy2e-/- Mouse Model of Leber Congenital Amaurosis-1. Human Gene Therapy. 2015 Aug 6. PubMed PMID: 26247368
  • Sanford L. Boye1, Antonette Bennett, Miranda L. Scalabrino, K. Tyler McCullough, Kim Van Vliet, Shreyasi Choudhury, Qing Ruan, James Peterson, Mavis Agbandje-McKenna, Shannon E. Boye. The Impact of Heparan Sulfate on Transduction of Retina by AAV Vectors. Journal of Virology. 2016; PMID: 26865709
  • Zaitseva M, McCullough KT, Cruz S, Thomas A, Diaz CG, Keilholz L, Grossi IM, Trost LC, Golding H. Post-challenge Administration of Brincidofovir Protects Normal and Immune-deficient Mice Reconstituted with Limited Numbers of T cells from Lethal Challenge with IHD-J-Luc Vaccinia Virus. Journal of Virology. 2015 Jan 14. pii: JVI.03340-14. PubMed PMID

Posters

  • McCullough KT, Gamlin P, Min SK, Peterson J, Cideciyan A, Alexander J, Hill J, Witherspoon C, Boye SL, Boye SE. Evaluating Relative Transduction Efficiencies of Optimized AAV Vectors in Mouse vs. Non-human Primate Retina. Celebration of Research, University of Florida, Gainesville, FL. 2015 Feb 9. Poster

Teaching

  • SSTP Seminar Series– Instructor in “Gene Therapy” course for advanced high school students

Service

  • Served in many domestic and international mission trips, including Mexico and Eleuthra, to repair homes, construct churches and build relationships with impoverished communities.
  • Regular Therapy Pet Volunteer at UF Health Hospital
  • Co-organizer of Foundation Fighting Blindness Jacksonville/Orlando chapter meeting “Gene Therapy 101”, University of Florida, September 2014